GM-CSF, Human：Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenit

產(chǎn)品型號：Z02695

更新時(shí)間：2025-11-06

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產(chǎn)品介紹

Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils. Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.

產(chǎn)品簡介

Species

Human

Protein Construction

Expressed with an N-terminal Met.

GM-CSF (Ala18-Glu144)
Accession # P04141

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 1 EU/μg of protein by gel clotting method

Biological Activity

ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 2.0 × 106 U/mg

Expression System

E. coli

Apparent Molecular Weight

~14 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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